Characterization of spermidine transport system in a cyanobacterium,synechocystis sp. PCC 6803.

Putrescine transport in a cyanobacterium Synechocystis sp. PCC 6803.

The transport of putrescine into a moderately salt tolerant cyanobacterium Synechocystis sp. PCC 6803 was characterized by measuring the uptake of radioactively-labeled putrescine. Putrescine transport showed saturation kinetics with an apparent K(m) of 92 +/- 10 microM and V(max) of 0.33 +/- 0.05 nmol/min/mg protein. The transport of putrescine was pH-dependent with highest activity at pH 7.0....

Thioredoxin peroxidase in the Cyanobacterium Synechocystis sp. PCC 6803.

The amino acid sequence deduced from the open reading frame designated sll0755 in Synechocystis sp. PCC 6803 is similar to the amino acid sequences of thioredoxin peroxidases from other organisms. In the present study, we found that a recombinant SLL0755 protein that was expressed in Escherichia coli was able to reduce H2O2 and tertiary butyl hydroperoxide with thioredoxin from E. coli as the e...

Deg/HtrA proteases of the cyanobacterium Synechocystis sp. PCC 6803 from biochemical characterization to their physiological functions

FLAVODIIRON2 and FLAVODIIRON4 proteins mediate an oxygen-dependent alternative electron flow in Synechocystis sp. PCC 6803 under CO2-limited conditions.

This study aims to elucidate the molecular mechanism of an alternative electron flow (AEF) functioning under suppressed (CO2-limited) photosynthesis in the cyanobacterium Synechocystis sp. PCC 6803. Photosynthetic linear electron flow, evaluated as the quantum yield of photosystem II [Y(II)], reaches a maximum shortly after the onset of actinic illumination. Thereafter, Y(II) transiently decrea...

Genetic Manipulation of the Cyanobacterium Synechocystis sp . PCC 6803 ' Development of Strains Lacking Photosystem I for the Analysis of Mutations in

We have taken a genetic approach to eliminating the presence of photosystem I (PSI) in site-directed mutants of photosystem I I (PSII) in the cyanobacterium Synechocystis sp. PCC 6803. By selecting under light-activated heterotrophic conditions, we have inactivated the psaA-psaS operon encoding the PSI reaction center proteins in cells containing deletions of the three psbA genes. We have also ...